至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

3D culture system to study collective migration in mammary organoid epithelium

STAR Protoc. 2021-08; 
Yunzhe Lu, Ruolan Deng, Huanyang You, Pengfei Lu
Products/Services Used Details Operation
Recombinant Proteins We have compared FGF10 provided by different manufacturers and have found that rmFGF10 (GenScript, Cat#Z03155) worked well. Get A Quote

摘要

We recently established an culture system in which mammary gland organoid undergoes directional migration in response to an FGF10 concentration gradient. Here, we describe a step-by-step protocol for preparing organoids, the setup of the 3D culture system, and the image acquisition approach. The technical difficulties in conducting the 3D migration assay are choosing epithelial organoids of appropriate sizes and manually paring organoids and beads pre-soaked in FGF10 within a desirable distance (∼100 μm). For complete details on the use and execution of this protocol, please refer to Lu et al. (2020).

关键词

Cell Biology, Cell culture, Developmental biology, Microscopy, Organoids